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Titre de page

Signalisation Cellulaire

Contenu de la page principale

Responsable : Dr Sabine Carpin

Professor assistants (lecturers):

Dr Sabine Carpin (HDR), Dr Christiane Depierreux, Dr François Héricourt, Dr Frédéric Lamblin (HDR)

 

Engineer and technician :

Dr Françoise Chefdor, Mélanie Larcher

 

Context

Earth's climate has changed throughout history and a global climate change is observed due to human activities. The last IPCC report confirms that extreme events and disasters as drought will become more intensive. In our regions, more intensive and frequent droughts will lead to reduced water availability to food crop, forest and populiculture.

Poplar culture which is economically important in the « Région Centre - Val de Loire », is typically restricted to well irrigated zones and will be consequently strongly affected by more drastic drought events. In the future, one major issue for poplar culture in this context will be to understand signaling pathways which contribute to perceive the stress leading to poplar adaptation to drought.

Such signaling pathways have been identified and one of them corresponds to a multi-step phosphorelay system (MSP). This kind of signaling pathway is composed by three partners : a receptor belonging to the Histidine-aspartate Kinase familly (HK), a shuttle protein belonging to the Histidine-containing Phosphotransfer protein family (HPt), and a third partner belonging to the Response Regulator protein family (RR) which comprises transcription factors (RR-B).

In poplar, we identified two HK proteins (HK1a and b) which could play a role in drought perception (Chefdor et al., 2006 ; Héricourt et al., 2016), 10 HPt proteins (HPt1 to 10) (Héricourt et al., 2013), 9 RR-B (Bertheau et al., 2012 ; 2013 ; 2015) and 10 RR-A (Chefdor et al., 2018).

A preferential partnership was identified between some of these proteins as shown in the Figure below.

Image
Fonctionnement MSP

Scheme of MSP potentialy involved in perception of drought in poplar

Image
MSP osmosensing peuplier

MSP partners and their relationship

 

Project

Cytokinins have been shown as negative regulators during drought (Tran et al., 2007) and it is now accepted that these phytohormones can play a role in plant acclimation/adaptation/tolerance during drought via MSP pathway (Li et al., 2016). Indeed, the key to understand abiotic stress tolerance resides in understanding the plant’s capacity to accelerate/maintain or repress growth (Dolferus 2014).

Consequently, plants constantly oscillate between normal or stress conditions thus between growth (acceleration or maintain) and stress tolerance (growth repression) via a shared MSP pathway between these two signals, cytokinin and drought.

However, understanding how both signals, drought and cytokinins, perceived by distinct receptors, can result in specific responses through an identical pool of HPt and RR-B proteins, remains to be determined.

Image
Question  HK

Our project focus on the study of MSP signaling pathways, trying to understand how those signaling pathway could permit plants to conserve specific responses to both signals and continue to grow during drought.

Our aims :

- To study HK1a and b roles and their operating mechanism

- To confirm the specificity between HPt2, 7 and 9 with HK1 compared to cytokinin receptors (HK2, 3 and 4).

- To study the roles of RR-B partners of HPt2, 7 and 9.

- To study RR-A roles in drought signaling.

 

Techniques

In vitro culture, hydroponic culture, protoplasts transformation, poplar agrotransformation, yeast two-hybrid, classical molecular biology techniques (RNA and DNA extraction, cloning techniques, sequencing, PCR, RT-PCR, western blot, etc …)

 

 

Poplar transformation

 

 

2H test RR18/HPt

 

Collaborations

- Laboratoire BBV, Université de Tours, France

- Centre de Biophysique Moléculaire (CBM), CNRS, Orléans, France

- Institute of Molecular and Cellular Biosciences, The University of Tokyo, Japon

- Dipartimento di Bioscienze e Territorio, Università degli Studi del Molise, Italie

 

Publications

2019

DAUDU D, KISIALA A, WERNER RIBEIRO C, MELIN C, PERROT L, CLASTRE M, COURDAVAULT V, PAPON N, OUDIN A, COURTOIS M, DUGE DE BERNONVILLE T, GAUCHER M, DEGRAVE A, LANOUE A, LANOTTE P, SCHOULER C, BRISSET M-N, EMERY RJN, PICHON O, CARPIN S, GIGLIOLI-GUIVARC'H N, CRECHE J, BESSEAU S, GLEVAREC G Setting-up a fast and reliable cytokinin biosensor based on a plant histidine kinase receptor expressed in Saccharomyces cerevisiae. Journal of Biotechnology, 289: 103-11

2018

Chefdor F., Héricourt F., Koudounas K., Carqueijeiro I., Courdavault V., Mascagni F., Bertheau L., Larcher M., Depierreux C., Lamblin F., Racchi M.L., and Carpin S. Highlighting type A RRs as potential regulators of the dkHK1 multistep phosphorelay pathway in Populus. Plant Science 277: 68-78.

2017

DAUDU D, ALLION E, LIESECKE F, PAPON N, COURDAVAULT V, DUGE DE BERNONVILLE T, MELIN C, OUDIN A, CLASTRE M, LANOUE A, COURTOIS M, PICHON O, GIRON D, CARPIN S, GIGLIOLI-GUIVARC'H, CRECHE J, BESSEAU S, GLEVAREC G (2017) CHASE-Containing Histidine Kinase Receptors in Apple Tree: From a Common Receptor Structure to Divergent Cytokinin Binding Properties and Specific Functions. Frontiers in Plant Science 8: 1614.

2016

Héricourt F., Chefdor F., Djeghdir I., Larcher M., Lafontaine F., Courdavault V., Auguin D., Coste F., Depierreux C., Tanigawa M., Maeda T., Glévarec G. and Carpin S. Functional Divergence of Poplar Histidine-Aspartate Kinase HK1 Paralogs in Response to Osmotic Stress. International Journal of Molecular Sciences 17: 2061-2079.

2015

Bertheau L., Djeghdir I., Fourreau E., Chefdor F., Glévarec G., Oudin A., Depierreux C., Morabito D., Brignolas F., Courdavault V., Héricourt F., Auguin D., Carpin S. Insights into the involvement of B-type RRs in the osmosensing signaling pathway in Populus. Plant Physiology and Biochemistry 94: 244-252.

2013

Bertheau L., Miranda M., Foureau E., Rojas Hoyos L.F., Chefdor F., Héricourt F., Depierreux C., Morabito D., Papon N., Clastre M., Scippa G.S., Brignolas F., Courdavault V., Carpin S. In planta validation of HK1 homodimerization and recruitment of preferential HPt downstream partners involved in poplar multistep phosphorelay systems. Plant Biosystems 147: 991-995.

Héricourt F.*, Chefdor F.*, Bertheau L., Tanigawa M., Maeda T., Guirimand G., Courdavault V., Larcher M., Depierreux C., Bénédetti H., Morabito D., Brignolas F., Carpin S. Characterization of histidine-aspartate kinase HK1 and identification of Histidine Phosphotransfer proteins as potential partners in a Populus multistep phosphorelay. Physiologia Plantarum 149: 188-199 (* co-premiers auteurs).

2012

Bertheau L., Chefdor F., Guirimand G., Courdavault V., Depierreux C., Morabito D., Brignolas F., Héricourt F., Carpin S. Identification of five B-type response regulators 1 as members of a multistep phosphorelay system interacting with Histidine-containing Phosphotransfer partners of Populus osmosensor, BMC Plant Biology 12: 241-254.

2006

Chefdor F., Bénédetti H., Depierreux C., Delmotte F., Morabito D., Carpin S. Osmotic stress sensing in Populus: Components identification of a phosphorelay system. FEBS Letters 580: 77-81.

 

Communications

  • May 2019

- Conférence du Dr Sabine Carpin

Programme Erasmus, Université de Florence (Italie)

"Multistep phosphorelay system, a signaling pathway between growth and drought tolerance"

- Conférence du Dr Roberto de Philippis, DAGRI, Université de Florence (Italie)

UFR Sciences et Techniques, Université d'Orléans

"Exploitation of microbial photosynthesis for environmentally sustainable biotechnological processes"

  • April 2019

- Conférence du Dr Sabine Carpin

EMBO Symposium, Grand Hotel, New Delhi (Inde)

"Stress sensing in tree species"

- Conférence du Dr Sabine Carpin

Workshop ICGEB, New Delhi (Inde)

"During drought, how to choose between growth and tolerance?"

- Conférence du Dr François Héricourt

Workshop ICGEB, New Delhi (Inde)

"Perception of drought in poplar: search for osmosensor and its ligand"

  • Novembre 2018

- Séminaire du Dr Françoise Chefdor

Equipe Signalisation Cellulaire, LBLGC, Université d'Orléans

"Highlighting type A RRs as potential regulators of the dkHK1 multistep phosphorelay pathway in Populus"

  • Septembre 2018

- Conférence du Pr Ashwani Pareek, Université Jawarharlal Nerhu, New Delhi (Inde)

Hôtel Dupanloup, Studium Orléans

"Towars improving abiotic stress tolerance in rice"

 

Students training by research

  • PhD thesis

- Inès Djeghdir (soutenue le 15 décembre 2016)

- Lucie Bertheau (soutenue le 19 décembre 2013)

- Françoise Chefdor (soutenue le 19 décembre 2006)

  • Master degree

- Master Agrosciences, Environnement, Territoires, Paysage, Forêt, Orléans

        Spécialité Biologie Intégrative et Changements Globaux

- Master Science du Vivant, Orléans

        Spécialité Biotechnologie, Biologie Moléculaire et Cellulaire

- Master Biologie des Plantes et des Micro-organismes, Biotechnologies, Bioprocédés, Montpellier

        Spécialité Biologie des Plantes

- Master Biologie, Agronomie, Santé, Rennes

        Spécialité Sciences Cellulaire et Moléculaire du Vivant

  • Undergraduate degree

- Licence 3 parcours BOPE et BMC, Orléans

- BTS Biotechnologie, Lycée Jacques Monod, St Jean de Braye

 

Grants

- Conseil régional du Centre : projet d’initiative académique 2013, SiSCyLi

- Conseil régional du Centre : projet « recherche » Appel Blanc 2008, SiSeP

- Conseil Général du Loiret : projet de recherche innovant 2008