Ph. MORIN¹, E. VANGREVELINGHE¹, S. MAYER¹, C. FRANCOIS^2*.

1 Institut de Chimie Organique et Analytique, URA CNRS 499, Université d’Orléans, BP 6759, 45067 Orléans cedex 2, France. 
2 Centre Analyse, TechnoParc, 6 rue du Carbone 45072 Orléans La Source cedex 2, France.

PHoEBuS, for a Microsoft environment, is an assistance software to prepare a buffer for capillary electrophoresis. It allows to predict its electrical, chemical and electrophoretic characteristics. The main applications of PHoEBuS software depend of the selected detection mode :

-Direct UV detection : the resolution of the separation depends on two physico-chemical parameters : pH and ionic strength. The PHoEBuS software allows the user to constitute a buffer at fixed pH and ionic strength.

- Indirect UV detection : the optimization and the detection sensitivity depend on buffer pH and on the chromophore ion concentration. The PHoEBuS software allows the user to constitute a buffer at fixed pH and concentration of co-ion by the user.

 Three working programs can be selected by the user:

            buffer preparation - fixed pH / ionic strength,

            buffer preparation - fixed pH / concentration,

            ionic compostion of an electrophoretic buffer.

*to whom correspondence should be addressed .

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01/03/00 12:26:56